Review



puc57 sgrna 647  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Addgene inc puc57 sgrna 647
    Puc57 Sgrna 647, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pm41655632-259-15-17
    Average 95 stars, based on 209 article reviews
    puc57 sgrna 647 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens.
    Article Snippet: Briefly, using the available sequencing information ofK. pneumoniaeATCC700603 strain, ~1- kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the mfd gene were synthesized by Genecust (Boynes, France). .. The two fragments were cloned on either side of the chloramphenicol resistance cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by Genecust. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and ligated to produce a “mfd-upstream”“CatR”-“mfd-downstream” KpnI-EcoRI fragment, which was then inserted between the KpnI and EcoRI sites of the pDG704 vector (Addgene).

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens
    Article Snippet: Briefly, using the available sequencing information of K. pneumoniae ATCC700603 strain, ~1-kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the mfd gene were synthesized by Genecust (Boynes, France). .. The two fragments were cloned on either side of the chloramphenicol resistance cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by Genecust. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and ligated to produce a “ mfd -upstream”-“CatR”-“ mfd -downstream” Kpn I- EcoR I fragment, which was then inserted between the Kpn I and EcoR I sites of the pDG704 vector (Addgene).

    Article Title: Chromatin-associated cohesin turns over extensively and forms new cohesive linkages during meiotic prophase
    Article Snippet: .. Following synthesis, GenScript cloned the construct into the pUC57 vector, verified the sequence, and subcloned the insertion into the w+attB vector (gift from Jeff Sekelsky, Addgene plasmid # 30326; http://n2t.net/addgene:30326 ). ..

    Article Title: Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.
    Article Snippet: The resulting amplicon was then cloned into the pCE2 TA/Blunt-Zero vector (C601-01, Vazyme, Nanjing, China). .. Similarly, the partial Muscovy duck reovirus (MDRV) σC gene fragment of 810 bp (GenBank accession number: KX831117.1) was synthesized and cloned into the PUC57 vector (Cat No. 54338, addgene, Waterton, MA, USA). ..

    Article Title: CRISPR/Cas9 Multiplex Genome Editing to Enhance Grain Lysine Concentration in a US Rice Cultivar
    Article Snippet: Rice (Oryza sativa L.) is a well-known staple crop providing daily calories to more than half of the world’s population.. Its nutritional value is reduced due to insignificant levels of essential amino acids.. Of the 20 amino acids, lysine is one of the most limiting factors, and low levels can lead to severe malnutrition issues affecting 25–30 % of children worldwide.

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens
    Article Snippet: Briefly, around 1-kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the targeted mfd gene were synthesized using the available sequencing information of K. pneumoniae ATCC700603 strain, by the Genecust company (Boynes, France). .. The two fragments upstream and downstream of the mfd gene were cloned at each side of the chloramphenicol cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by the Genecust company. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and assembled by ligation to produce a “ mfd -upstream”-“CatR”-“ mfd -downstream” Kpn I- EcoR I fragment, which was then inserted between the Kpn I and EcoR I sites of the pDG704 vector (Addgene).

    Article Title: Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a
    Article Snippet: The resulting amplicon was then cloned into the pCE2 TA/Blunt-Zero vector (C601-01, Vazyme, Nanjing, China). .. Similarly, the partial Muscovy duck reovirus (MDRV) σC gene fragment of 810 bp (GenBank accession number: KX831117.1) was synthesized and cloned into the PUC57 vector (Cat No. 54338, addgene, Waterton, MA, USA). ..

    Plasmid Preparation:

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens.
    Article Snippet: Briefly, using the available sequencing information ofK. pneumoniaeATCC700603 strain, ~1- kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the mfd gene were synthesized by Genecust (Boynes, France). .. The two fragments were cloned on either side of the chloramphenicol resistance cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by Genecust. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and ligated to produce a “mfd-upstream”“CatR”-“mfd-downstream” KpnI-EcoRI fragment, which was then inserted between the KpnI and EcoRI sites of the pDG704 vector (Addgene).

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens
    Article Snippet: Briefly, using the available sequencing information of K. pneumoniae ATCC700603 strain, ~1-kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the mfd gene were synthesized by Genecust (Boynes, France). .. The two fragments were cloned on either side of the chloramphenicol resistance cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by Genecust. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and ligated to produce a “ mfd -upstream”-“CatR”-“ mfd -downstream” Kpn I- EcoR I fragment, which was then inserted between the Kpn I and EcoR I sites of the pDG704 vector (Addgene).

    Article Title: Chromatin-associated cohesin turns over extensively and forms new cohesive linkages during meiotic prophase
    Article Snippet: .. Following synthesis, GenScript cloned the construct into the pUC57 vector, verified the sequence, and subcloned the insertion into the w+attB vector (gift from Jeff Sekelsky, Addgene plasmid # 30326; http://n2t.net/addgene:30326 ). ..

    Article Title: CRISPR/Cas9 Multiplex Genome Editing to Enhance Grain Lysine Concentration in a US Rice Cultivar
    Article Snippet: Rice (Oryza sativa L.) is a well-known staple crop providing daily calories to more than half of the world’s population.. Its nutritional value is reduced due to insignificant levels of essential amino acids.. Of the 20 amino acids, lysine is one of the most limiting factors, and low levels can lead to severe malnutrition issues affecting 25–30 % of children worldwide.

    Article Title: A Novel Drosophila-based Drug Repurposing Platform Identified Fingolimod As a Potential Therapeutic for TDP-43 Proteinopathy.
    Article Snippet: Pathogenic changes to TAR DNA-binding protein 43 (TDP-43) leading to alteration of its homeostasis are a common feature shared by several progressive neurodegenerative diseases for which there is no effective therapy.. Here, we developed Drosophila lines expressing either wild type TDP-43 (WT) or that carrying an Amyotrophic Lateral Sclerosis /Frontotemporal Lobar Degeneration-associating G384C mutation that recapitulate several aspects of the TDP-43 pathology.. To identify potential therapeutics for TDP-43-related diseases, we implemented a drug repurposing strategy that involved three consecutive steps.

    Article Title: An anti-virulence drug targeting the evolvability protein Mfd protects against infections with antimicrobial resistant ESKAPE pathogens
    Article Snippet: Briefly, around 1-kb region upstream (region 2056347 to 2057137) and downstream (region 2060189 to 2060810) of the targeted mfd gene were synthesized using the available sequencing information of K. pneumoniae ATCC700603 strain, by the Genecust company (Boynes, France). .. The two fragments upstream and downstream of the mfd gene were cloned at each side of the chloramphenicol cassette (CatR) from the pDK3 plasmid (Addgene) into the pUC57 vector (Addgene) by the Genecust company. .. The synthesized DNA fragments and the CatR cassette were digested with the appropriate enzymes and assembled by ligation to produce a “ mfd -upstream”-“CatR”-“ mfd -downstream” Kpn I- EcoR I fragment, which was then inserted between the Kpn I and EcoR I sites of the pDG704 vector (Addgene).

    Construct:

    Article Title: Chromatin-associated cohesin turns over extensively and forms new cohesive linkages during meiotic prophase
    Article Snippet: .. Following synthesis, GenScript cloned the construct into the pUC57 vector, verified the sequence, and subcloned the insertion into the w+attB vector (gift from Jeff Sekelsky, Addgene plasmid # 30326; http://n2t.net/addgene:30326 ). ..

    Article Title: CRISPR/Cas9 Multiplex Genome Editing to Enhance Grain Lysine Concentration in a US Rice Cultivar
    Article Snippet: Rice (Oryza sativa L.) is a well-known staple crop providing daily calories to more than half of the world’s population.. Its nutritional value is reduced due to insignificant levels of essential amino acids.. Of the 20 amino acids, lysine is one of the most limiting factors, and low levels can lead to severe malnutrition issues affecting 25–30 % of children worldwide.

    Sequencing:

    Article Title: Chromatin-associated cohesin turns over extensively and forms new cohesive linkages during meiotic prophase
    Article Snippet: .. Following synthesis, GenScript cloned the construct into the pUC57 vector, verified the sequence, and subcloned the insertion into the w+attB vector (gift from Jeff Sekelsky, Addgene plasmid # 30326; http://n2t.net/addgene:30326 ). ..

    Article Title: A Novel Drosophila-based Drug Repurposing Platform Identified Fingolimod As a Potential Therapeutic for TDP-43 Proteinopathy.
    Article Snippet: Pathogenic changes to TAR DNA-binding protein 43 (TDP-43) leading to alteration of its homeostasis are a common feature shared by several progressive neurodegenerative diseases for which there is no effective therapy.. Here, we developed Drosophila lines expressing either wild type TDP-43 (WT) or that carrying an Amyotrophic Lateral Sclerosis /Frontotemporal Lobar Degeneration-associating G384C mutation that recapitulate several aspects of the TDP-43 pathology.. To identify potential therapeutics for TDP-43-related diseases, we implemented a drug repurposing strategy that involved three consecutive steps.

    Synthesized:

    Article Title: Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a.
    Article Snippet: The resulting amplicon was then cloned into the pCE2 TA/Blunt-Zero vector (C601-01, Vazyme, Nanjing, China). .. Similarly, the partial Muscovy duck reovirus (MDRV) σC gene fragment of 810 bp (GenBank accession number: KX831117.1) was synthesized and cloned into the PUC57 vector (Cat No. 54338, addgene, Waterton, MA, USA). ..

    Article Title: CRISPR/Cas9 Multiplex Genome Editing to Enhance Grain Lysine Concentration in a US Rice Cultivar
    Article Snippet: Rice (Oryza sativa L.) is a well-known staple crop providing daily calories to more than half of the world’s population.. Its nutritional value is reduced due to insignificant levels of essential amino acids.. Of the 20 amino acids, lysine is one of the most limiting factors, and low levels can lead to severe malnutrition issues affecting 25–30 % of children worldwide.

    Article Title: A Novel Drosophila-based Drug Repurposing Platform Identified Fingolimod As a Potential Therapeutic for TDP-43 Proteinopathy.
    Article Snippet: Pathogenic changes to TAR DNA-binding protein 43 (TDP-43) leading to alteration of its homeostasis are a common feature shared by several progressive neurodegenerative diseases for which there is no effective therapy.. Here, we developed Drosophila lines expressing either wild type TDP-43 (WT) or that carrying an Amyotrophic Lateral Sclerosis /Frontotemporal Lobar Degeneration-associating G384C mutation that recapitulate several aspects of the TDP-43 pathology.. To identify potential therapeutics for TDP-43-related diseases, we implemented a drug repurposing strategy that involved three consecutive steps.

    Article Title: Development of a Rapid Visual Detection Assay for Duck Tembusu Virus Using RT-LAMP-CRISPR/Cas12a
    Article Snippet: The resulting amplicon was then cloned into the pCE2 TA/Blunt-Zero vector (C601-01, Vazyme, Nanjing, China). .. Similarly, the partial Muscovy duck reovirus (MDRV) σC gene fragment of 810 bp (GenBank accession number: KX831117.1) was synthesized and cloned into the PUC57 vector (Cat No. 54338, addgene, Waterton, MA, USA). ..

    Mutagenesis:

    Article Title: A Novel Drosophila-based Drug Repurposing Platform Identified Fingolimod As a Potential Therapeutic for TDP-43 Proteinopathy.
    Article Snippet: Pathogenic changes to TAR DNA-binding protein 43 (TDP-43) leading to alteration of its homeostasis are a common feature shared by several progressive neurodegenerative diseases for which there is no effective therapy.. Here, we developed Drosophila lines expressing either wild type TDP-43 (WT) or that carrying an Amyotrophic Lateral Sclerosis /Frontotemporal Lobar Degeneration-associating G384C mutation that recapitulate several aspects of the TDP-43 pathology.. To identify potential therapeutics for TDP-43-related diseases, we implemented a drug repurposing strategy that involved three consecutive steps.



    Similar Products

    99
    New England Biolabs puc57 vector 357
    Puc57 Vector 357, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/EcoRI/10__1016_slash_j__omtn__2025__102809-135-8-14
    Average 99 stars, based on 1 article reviews
    puc57 vector 357 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Sangon Biotech puc57 vectors
    Puc57 Vectors, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/orf1+pcv2+pcv2+plasmid+pmd18+positive/pm42243997-82-19-22
    Average 86 stars, based on 1 article reviews
    puc57 vectors - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Sangon Biotech puc57 vector
    Puc57 Vector, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/orf1+pcv2+pcv2+plasmid+pmd18+positive/pm41812557-90-29-16
    Average 86 stars, based on 1 article reviews
    puc57 vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Addgene inc puc57 sgrna 647
    Puc57 Sgrna 647, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pm41655632-259-15-17
    Average 95 stars, based on 1 article reviews
    puc57 sgrna 647 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Addgene inc sgrna expression vector
    Sgrna Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pmc12911573-45-5-9
    Average 95 stars, based on 1 article reviews
    sgrna expression vector - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    86
    Azenta puc57 kan gw ok vector
    Puc57 Kan Gw Ok Vector, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/puc57/pmc13001375-55-13-16
    Average 86 stars, based on 1 article reviews
    puc57 kan gw ok vector - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    95
    Addgene inc bbsi sites
    Bbsi Sites, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pm41383737-67-16-21
    Average 95 stars, based on 1 article reviews
    bbsi sites - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    Addgene inc puc57 sgrna
    Puc57 Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc57+vector/pUC57-sgRNA+expression+vector+(Plasmid+%2351132)/pmc12884795-207-1-3
    Average 95 stars, based on 1 article reviews
    puc57 sgrna - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results